Pathogen Tests

The Regional Pulse Crop Diagnostic Laboratory Services

Seed is sampled for Ascochyta-Plus and nematode testing according to ISTA (International Seed Testing Association) protocols and guidelines.  Samples are initially handled by the Montana State Seed lab, an AOSA lab (Association of Official Seed Analysts).
 

1. Ascochyta-Plus: $180/sample (Chickpea, Lentil, and Pea)

   In this package, we test for Ascochyta (now Didymella) pathogens and five other fungal pathogens of economic importance, as listed below:

    

   Pathogens	Disease
   Ascochyta (now Didymella) spp	Leaf, stem, & pod blight
   Botrytis spp.	Grey mold
   Colletotrichum spp.	Anthracnose
   Sclerotina spp.	White mold
   Stemphylium spp.	Leaf blight

    

   Price: $180/sample

   Quantity of Seeds for test:½ lb. for lentil, 1lb. for dry pea, 2 lbs. for chickpea.

   Test Time:It usually takes 7-10 days, after we have received the sample.

   Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

   Method:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

           % Infection:100*(Number of seeds from with a fungal pathogen /Total number of seeds)

    

2. Smart-B: Costs: $350 (Chickpea, Lentil, and Pea)

   The package includes Ascochyta-Plus and bacterial blight agents. This is a good choice if you are only concerned about fungal and bacterial blight diseases.

    

   Pathogens	Disease
   Ascochyta (now Didymella) spp	Leaf, stem, & pod blight
   Botrytis spp.	Grey mold
   Colletotrichum spp.	Anthracnose
   Sclerotinia spp.	White mold
   Stemphylium spp.	Leaf blight
   Psuedomonas syringae pv pisi & pv syringae	Bacterial blight

    

   Price: $350/sample

   Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.

   Test Time:It usually takes 10-12 days, after we have received the sample.

   Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

   Methods

   Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

   Infection: 100*(Number of seeds from with Ascochyta /Total number of seeds)

          Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas  syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).

    

3. Smart-V: Costs: $330 (Chickpea, Lentil, and Pea)

   The package includes Ascochyta-Plus and Pea seedborne mosaic virus (PSbMV) tests. We are no longer testing for Pea enation mosaic virus (PEMV) because its seed transmissibility is in doubt. This is a good choice if you are only concerned about fungal diseases and Pea seedborne mosaic virus.

    

   Pathogens	Disease
   Ascochyta (now Didymella) spp	Leaf, stem, & pod blight
   Botrytis spp.	Grey mold
   Colletotrichum spp.	Anthracnose
   Sclerotinia spp.	White mold
   Stemphylium spp.	Leaf blight
   Pea seedborne mosaic virus (PSbMV)	Empty pods, seed blemishes, reduced seed yield

    

   Price: $320/sample

   Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.

   Test Time:It usually takes 10-12 days, after we have received the sample.

   Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

   Methods

   Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

   % Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)

   Virus:Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent assay (ELISA), and maximum likelihood method is used to estimate infection rate (%).

    

4. Comprehensive ($450) (Chickpea, Lentil, and Pea)

   This package includes tests for AscochytaPlus, Pea seedborne mosaic virus (PSbMV), and bacterial blight agents (Psuedomonas syringae pv pisi and pv syringae).

   We recommend this package the most!

    

   Pathogens	Disease
   Ascochyta (now Didymella) spp	Leaf, stem, & pod blight
   Botrytis spp.	Grey mold
   Colletotrichum spp.	Anthracnose
   Sclerotinia spp.	White mold
   Stemphylium spp.	Leaf blight
   Pea seedborne mosaic virus (PSbMV)	Empty pods, seed blemishes, reduced seed yield
   Psuedomonas syringae pv pisi & pv syringae	Bacterial blight

    

   Price: $500/sample

   Quantity of Seeds for test:2.5 lb. for lentil, 5 lb. for dry pea and chickpea.

   Test Time:It usually takes 10-12 days, after we have received the sample.

   Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

   Methods

   Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

   % Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)

         Virus: Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent   assay (ELISA), and maximum likelihood method is used to estimate infection rate (%). For bacterial blight test, 5 subsamples of 1000 each are tested for the presence of Psuedomonas syringae pathovars pisi and syringae.

         Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).

    

5. Nematode tests ($35) (all crops)

   We offer test for seedborne or contaminated nematodes of Ditylenchus and

   Heterdoera spp.

   Test	Seed Quantity	Price/sample
   Ditylenchus spp (stem and bulb nematodes) and Heterodera spp.(pea cyst nematodes)	1 pound	$35

    

   Note:Test Time: It usually takes 2 days from the date of receipt to complete test. Rush Fee: $35 per sample for nematode test. We discourage Rush, so send your samples ahead of time! Rush means your sample will be placed ahead of other non- rush samples on the queue

    

6. Blackleg Testing ($150) (Canola)

    

   Pathogens	Disease
   Leptosphaeria maculans (Phoma lingam)	Aggressive blackleg (phoma stem canker)
   Leptosphaeria biglobosa (Phoma herbarum)	Non-aggressive blackleg (phoma stem canker)

   Price: $150/sample

   Quantity of Seeds for test:1lb of canola seeds

   Test Time:It usually takes 12-14 days, after we have received the sample.

   Rush Fee:$100 per sample per test. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

   Method

   Fungi:Canola: 1000 seeds treated with 0.2% 2,4-Dichlorophenoxyacetate (2,4D) to prevent seed germination. Seeds will be placed on moistened blotters in petri plate; 50 seeds/plate

   % Infect: 100*(Number of seeds from with each blackleg pathogen /Total number of seeds) and incubated at 20⁰ C for 11 days.

   Differentiation: Leptosphaeria maculans versus Leptosphaeria biglobosa Morphology: Size of pycnidia, presence of papilla, and the color of pycnidia exudate ISTA 7-004. Detection of Phoma lignam

    

7. Other Individual Pathogen Tests (Chickpea, lentil, and dry peas)

We also offer individual pathogen test included in the Price list below:

 Pathogen Tests July 2018 (PDF)