Pathogen Tests
The Regional Pulse Crop Diagnostic Laboratory Services
New Reduced Prices and Services Effective from 15 July 2018!!!
More tests for less price!
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Ascochyta-Plus: $180/sample (Chickpea, Lentil, and Pea)
In this package, we test for Ascochyta (now Didymella) pathogens and five other fungal pathogens of economic importance, as listed below:
Pathogens
Disease
Ascochyta (now Didymella) spp
Leaf, stem, & pod blight
Botrytis spp.
Grey mold
Colletotrichum spp.
Anthracnose
Sclerotina spp.
White mold
Stemphylium spp.
Leaf blight
Price: $180/sample
Quantity of Seeds for test:½ lb. for lentil, 1lb. for dry pea, 2 lbs. for chickpea.
Test Time:It usually takes 7-10 days, after we have received the sample.
Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.
Method:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).
% Infection:100*(Number of seeds from with a fungal pathogen /Total number of seeds)
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Smart-B: Costs: $350 (Chickpea, Lentil, and Pea)
The package includes Ascochyta-Plus and bacterial blight agents. This is a good choice if you are only concerned about fungal and bacterial blight diseases.
Pathogens
Disease
Ascochyta (now Didymella) spp
Leaf, stem, & pod blight
Botrytis spp.
Grey mold
Colletotrichum spp.
Anthracnose
Sclerotinia spp.
White mold
Stemphylium spp.
Leaf blight
Psuedomonas syringae pv pisi & pv
syringae
Bacterial blight
Price: $350/sample
Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.
Test Time:It usually takes 10-12 days, after we have received the sample.
Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.
Methods
Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).
Infection: 100*(Number of seeds from with Ascochyta /Total number of seeds)
Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).
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Smart-V: Costs: $330 (Chickpea, Lentil, and Pea)
The package includes Ascochyta-Plus and Pea seedborne mosaic virus (PSbMV) tests. We are no longer testing for Pea enation mosaic virus (PEMV) because its seed transmissibility is in doubt. This is a good choice if you are only concerned about fungal diseases and Pea seedborne mosaic virus.
Pathogens
Disease
Ascochyta (now Didymella) spp
Leaf, stem, & pod blight
Botrytis spp.
Grey mold
Colletotrichum spp.
Anthracnose
Sclerotinia spp.
White mold
Stemphylium spp.
Leaf blight
Pea seedborne mosaic virus
(PSbMV)
Empty pods, seed blemishes, reduced seed yield
Price: $320/sample
Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.
Test Time:It usually takes 10-12 days, after we have received the sample.
Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.
Methods
Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).
% Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)
Virus:Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent assay (ELISA), and maximum likelihood method is used to estimate infection rate (%).
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Comprehensive ($450) (Chickpea, Lentil, and Pea)
This package includes tests for AscochytaPlus, Pea seedborne mosaic virus (PSbMV), and bacterial blight agents (Psuedomonas syringae pv pisi and pv syringae).
We recommend this package the most!
Pathogens
Disease
Ascochyta (now Didymella) spp
Leaf, stem, & pod blight
Botrytis spp.
Grey mold
Colletotrichum spp.
Anthracnose
Sclerotinia spp.
White mold
Stemphylium spp.
Leaf blight
Pea seedborne mosaic virus (PSbMV)
Empty pods, seed blemishes, reduced seed yield
Psuedomonas syringae pv pisi & pv syringae
Bacterial blight
Price: $500/sample
Quantity of Seeds for test:2.5 lb. for lentil, 5 lb. for dry pea and chickpea.
Test Time:It usually takes 10-12 days, after we have received the sample.
Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.
Methods
Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).
% Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)
Virus: Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent assay (ELISA), and maximum likelihood method is used to estimate infection rate (%). For bacterial blight test, 5 subsamples of 1000 each are tested for the presence of Psuedomonas syringae pathovars pisi and syringae.
Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).
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Nematode tests ($35) (all crops)
We offer test for seedborne or contaminated nematodes of Ditylenchus and
Heterdoera spp.
Test
Seed Quantity
Price/sample
Ditylenchus spp (stem and bulb nematodes) and Heterodera spp.(pea cyst nematodes)
1 pound
$35
Note:Test Time: It usually takes 2 days from the date of receipt to complete test. Rush Fee: $35 per sample for nematode test. We discourage Rush, so send your samples ahead of time! Rush means your sample will be placed ahead of other non- rush samples on the queue
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Blackleg Testing ($150) (Canola)
Pathogens
Disease
Leptosphaeria maculans (Phoma lingam)
Aggressive blackleg (phoma stem canker)
Leptosphaeria biglobosa (Phoma herbarum)
Non-aggressive blackleg (phoma stem canker)
Price: $150/sample
Quantity of Seeds for test:1lb of canola seeds
Test Time:It usually takes 12-14 days, after we have received the sample.
Rush Fee:$100 per sample per test. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.
Method
Fungi:Canola: 1000 seeds treated with 0.2% 2,4-Dichlorophenoxyacetate (2,4D) to prevent seed germination. Seeds will be placed on moistened blotters in petri plate; 50 seeds/plate
% Infect: 100*(Number of seeds from with each blackleg pathogen /Total number of seeds) and incubated at 20⁰ C for 11 days.
Differentiation: Leptosphaeria maculans versus Leptosphaeria biglobosa Morphology: Size of pycnidia, presence of papilla, and the color of pycnidia exudate ISTA 7-004. Detection of Phoma lignam
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Other Individual Pathogen Tests (Chickpea, lentil, and dry peas)
We also offer individual pathogen test included in the Price list below:
Test for Individual Fungal Agent(s) of: |
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Ascochyta/Mycospharella spp |
$180 for oneor more of these fungal pathogen tests. |
Aspergillus spp. |
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Botrytis spp. |
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Colletotrichum spp. |
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Fusarium spp. |
Rhizoctonia solani |
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Sclerotinia spp. |
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Stemphylium |
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Individual Virus Tests |
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Pea seed-borne mosaic virus (PSbMV) |
$180 |
Pea enation mosaic virus (PEMV) |
$150 |
Alfalfa mosaic virus (AMV) |
$150 |
Cucumber mosaic virus (CMV) |
$150 |
Bean yellow mosaic virus (BYMV) |
$150 |
Barley stripe mosaic virus (BSMV) |
$100 |
Bacterial Test |
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Pseudomonas syringae pv pisi |
$200 |
Pseudomonas syringae pv syringae |
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Phytoplasma Test |
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Aster yellows phytoplasma |
$150 |
Pathogen Tests July 2018 (PDF)