The Regional Pulse Crop Diagnostic Laboratory Services

New Reduced Prices and Services Effective from 15 July 2018!!!
More tests for less price!

 

  1. Ascochyta-Plus: $180/sample (Chickpea, Lentil, and Pea)

    In this package, we test for Ascochyta (now Didymella) pathogens and five other fungal pathogens of economic importance, as listed below:

     

    Pathogens

    Disease

    Ascochyta (now Didymella) spp

    Leaf, stem, & pod blight

    Botrytis spp.

    Grey mold

    Collectotrichum spp.

    Anthracnose

    Fusarium spp.

    Wilt and root rot

    Sclerotina spp.

    White mold

    Stemphylium spp.

    Leaf blight

     

    Price: $180/sample

    Quantity of Seeds for test:½ lb. for lentil, 1lb. for dry pea, 2 lbs. for chickpea.

    Test Time:It usually takes 7-10 days, after we have received the sample.

    Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

    Method:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

            % Infection:100*(Number of seeds from with a fungal pathogen /Total number of seeds)

     

  2. Smart-B: Costs: $350 (Chickpea, Lentil, and Pea)

    The package includes Ascochyta-Plus and bacterial blight agents. This is a good choice if you are only concerned about fungal and bacterial blight diseases.

     

    Pathogens

    Disease

    Ascochyta (now Didymella) spp

    Leaf, stem, & pod blight

    Botrytis spp.

    Grey mold

    Collectotrichum spp.

    Anthracnose

    Fusarium spp.

    Wilt and root rot

    Sclerotinia spp.

    White mold

    Stemphylium spp.

    Leaf blight

    Psuedomonas syringae pv pisi & pv

    syringae

    Bacterial blight

     

    Price: $350/sample

    Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.

    Test Time:It usually takes 10-12 days, after we have received the sample.

    Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

    Methods

    Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

    Infection: 100*(Number of seeds from with Ascochyta /Total number of seeds)

           Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas  syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).

     

  3. Smart-V: Costs: $330 (Chickpea, Lentil, and Pea)

    The package includes Ascochyta-Plus and Pea seedborne mosaic virus (PSbMV) tests. We are no longer testing for Pea enation mosaic virus (PEMV) because its seed transmissibility is in doubt. This is a good choice if you are only concerned about fungal diseases and Pea seedborne mosaic virus.

     

    Pathogens

    Disease

    Ascochyta (now Didymella) spp

    Leaf, stem, & pod blight

    Botrytis spp.

    Grey mold

    Collectotrichum spp.

    Anthracnose

    Fusarium spp.

    Wilt and root rot

    Sclerotinia spp.

    White mold

    Stemphylium spp.

    Leaf blight

    Pea seedborne mosaic virus

    (PSbMV)

    Empty pods, seed blemishes, reduced seed yield

     

    Price: $320/sample

    Quantity of Seeds for test:1.5 lb. for lentil, 2.5 lb. for dry pea and chickpea.

    Test Time:It usually takes 10-12 days, after we have received the sample.

    Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

    Methods

    Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

    % Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)

    Virus:Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent assay (ELISA), and maximum likelihood method is used to estimate infection rate (%).

     

  4. Comprehensive ($450) (Chickpea, Lentil, and Pea)

    This package includes tests for AscochytaPlus, Pea seedborne mosaic virus (PSbMV), and bacterial blight agents (Psuedomonas syringae pv pisi and pv syringae).

    We recommend this package the most!

     

    Pathogens

    Disease

    Ascochyta (now Didymella) spp

    Leaf, stem, & pod blight

    Botrytis spp.

    Grey mold

    Collectotrichum spp.

    Anthracnose

    Fusarium spp.

    Wilt and root rot

    Sclerotinia spp.

    White mold

    Stemphylium spp.

    Leaf blight

    Pea seedborne mosaic virus (PSbMV)

    Empty pods, seed blemishes, reduced seed yield

    Psuedomonas syringae pv pisi & pv syringae

    Bacterial blight

     

    Price: $500/sample

    Quantity of Seeds for test:2.5 lb. for lentil, 5 lb. for dry pea and chickpea.

    Test Time:It usually takes 10-12 days, after we have received the sample.

    Rush Fee:$100 per sample per Ascochyta-Plus test only. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

    Methods

    Fungi:Chickpea, dry pea, and lentil: 400 seeds are plated in potato dextrose agar (PDA).

    % Infection:100*(Number of seeds from with Ascochyta /Total number of seeds)

          Virus: Twenty subsamples of 100 seeds each are test for the presence of the virus in Enzyme immunosorbent   assay (ELISA), and maximum likelihood method is used to estimate infection rate (%). For bacterial blight test, 5 subsamples of 1000 each are tested for the presence of Psuedomonas syringae pathovars pisi and syringae.

          Bacterial blight test: Five subsamples of 1000 seeds each are test for the presence of Psuedomonas syringae pathovars pisi and syringae, and maximum likelihood method is used to estimate infection rate (%).

     

  5. Nematode tests ($35) (all crops)

    We offer test for seedborne or contaminated nematodes of Ditylenchus and

    Heterdoera spp.

    Test

    Seed Quantity

    Price/sample

    Ditylenchus spp (stem and bulb nematodes) and Heterodera spp.(pea cyst nematodes)

    1 pound

    $35

     

    Note:Test Time: It usually takes 2 days from the date of receipt to complete test. Rush Fee: $35 per sample for nematode test. We discourage Rush, so send your samples ahead of time! Rush means your sample will be placed ahead of other non- rush samples on the queue

     

  6. Blackleg Testing ($150) (Canola)

     

    Pathogens

    Disease

    Leptosphaeria maculans (Phoma lingam)

    Aggressive blackleg (phoma stem canker)

    Leptosphaeria biglobosa (Phoma herbarum)

    Non-aggressive blackleg (phoma stem canker)

    Price: $150/sample

    Quantity of Seeds for test:1lb of canola seeds

    Test Time:It usually takes 12-14 days, after we have received the sample.

    Rush Fee:$100 per sample per test. We discourage Rush; so, send your samples ahead of time! Rush means your sample will be placed ahead of other non-rush samples on the queue.

    Method

    Fungi:Canola: 1000 seeds treated with 0.2% 2,4-Dichlorophenoxyacetate (2,4D) to prevent seed germination. Seeds will be placed on moistened blotters in petri plate; 50 seeds/plate

    % Infect: 100*(Number of seeds from with each blackleg pathogen /Total number of seeds) and incubated at 20C for 11 days.

    Differentiation: Leptosphaeria maculans versus Leptosphaeria biglobosa Morphology: Size of pycnidia, presence of papilla, and the color of pycnidia exudate ISTA 7-004. Detection of Phoma lignam

     

  7. Other Individual Pathogen Tests (Chickpea, lentil, and dry peas)

We also offer individual pathogen test included in the Price list below:

Test for Individual Fungal Agent(s) of:

 

Ascochyta/Mycospharella spp

$180 for oneor more of these fungal pathogen

tests.

Aspergillus spp.

Botrytis spp.

Collectotrichum spp.

Fusarium spp.

Rhizoctonia solani

 

Sclerotinia spp.

Stemphylium

 

Individual Virus Tests

 

Pea seed-borne mosaic virus (PSbMV)

$180

Pea enation mosaic virus (PEMV)

$150

Alfalfa mosaic virus (AMV)

$150

Cucumber mosaic virus (CMV)

$150

Bean yellow mosaic virus (BYMV)

$150

Barley stripe mosaic virus (BSMV)

$100

Bacterial Test

 

Pseudomonas syringae pv pisi

 

$200

Pseudomonas syringae pv syringae

Phytoplasma Test

 

Aster yellows phytoplasma

$150

 

 

 Pathogen Tests July 2018 (PDF)